Effect of washing, soaking and pH in combination with ultrasound on enzymatic rancidity, phytic acid, heavy metals and coliforms of rice bran

Abstract Simultaneous reduction in activity of fat destabilizing enzymes (lipase and lipoxygenase), contaminants heavy metals (As, Cd, Pb, Hg), antinutrient phytic acid hazardous coliforms rice bran was investigated. Application washing, soaking the washed sample at different pH values (2, 6 9) alone or combination with ultrasonication were examined. While washing beneficial, its low efficiency acquired further treatment, which prevailed by application acidic ultrasound (28 kHz) treatments. Free fatty acids peroxide value, as indicators activity, implied effectiveness treatments adverse impact sonication on value. Remarkably, dominant Pb Zn) synergistically facilitated sonication. Coliforms growth inhibited 2 even absence ultrasonic treatment. Evidently, is a practical approach to improve stability safety.